What would be the best experiment to do next to determine if complete hexokinase gene expression is temperature dependent?
A)Measure the amount of hexokinase mRNA in cells at different temperatures.
B)Measure the rate of transcription of the hexokinase gene in cells at different temperatures.
C)Measure the amount of mRNA that is broken down in cells at different temperatures.
D)Measure the amount of hexokinase protein in cells at different temperatures.

Question

Quizplus · Accepted Answer

Measuring the amount of hexokinase protein in cells at different temperatures would directly determine if the complete expression of the hexokinase gene is temperature dependent. This approach would be more specific than measuring mRNA levels because mRNA levels may not always correlate with protein levels due to post-transcriptional regulation.

What Would Be the Best Experiment to Do Next to Determine