The steps involved in a single PCR cycle, in the correct order, are
A) cool to allow DNA strands to anneal, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
B) heat to denature DNA strands, cool to allow primer to anneal, allow DNA polymerase to copy DNA.
C) heat to denature DNA strands and for primer to anneal, cool to allow DNA polymerase to copy DNA.
D) cool to denature DNA strands, heat to allow primer to anneal, cool to allow DNA polymerase to copy DNA.
E) cool to allow primer to anneal, heat to denature DNA strands, cool to allow DNA polymerase to copy DNA.

Question

Quizplus · Accepted Answer

The correct order of steps in a PCR cycle is: 1) heating the reaction mixture to denature the DNA strands, 2) cooling it to allow the primers to anneal to the single-stranded DNA, and 3) maintaining a suitable temperature for DNA polymerase to synthesize new DNA strands.

The Steps Involved in a Single PCR Cycle, in the Correct