The size of the amplified DNA fragment generated during PCR is determined by
A) how many cycles are performed.
B) the size of the template DNA.
C) the location to which the primers anneal.
D) how much Taq polymerase is used.
E) the time for each cycle.

Question

The size of the amplified DNA fragment generated during PCR is determined by
A) how many cycles are performed.
B) the size of the template DNA.
C) the location to which the primers anneal.
D) how much Taq polymerase is used.
E) the time for each cycle.

Quizplus · Accepted Answer

The size of the amplified DNA fragment is primarily determined by the location to which the primers anneal. The size of the template DNA and the amount of Taq polymerase used may influence the yield of the amplification, but not the specific size of the amplified product. The number of cycles and the time for each cycle may affect the amount of amplification but not the size of the DNA fragment.

The Size of the Amplified DNA Fragment Generated During PCR