The colonies of interest in this case are the blue-green presumptive Shigella colonies. However, growth on hektoen enteric agar is not sufficient for definitive identification. Further tests are needed. First, the lab technicians need to prepare larger numbers of Shigella from your patient's stool sample, and then they need to ensure that they are culturing a single species. The methods used to do this are ________, followed by ________.
A) streak plating; pour plates
B) enrichment culture; Gram staining
C) Gram staining; enrichment culture
D) Gram staining; capsule staining
E) enrichment culture; streak plating

Question

The colonies of interest in this case are the blue-green presumptive Shigella colonies. However, growth on hektoen enteric agar is not sufficient for definitive identification. Further tests are needed. First, the lab technicians need to prepare larger numbers of Shigella from your patient's stool sample, and then they need to ensure that they are culturing a single species. The methods used to do this are ________, followed by ________. 
A) streak plating; pour plates
B) enrichment culture; Gram staining
C) Gram staining; enrichment culture
D) Gram staining; capsule staining
E) enrichment culture; streak plating

Quizplus · Accepted Answer

Enrichment culture is the method used to obtain larger numbers of Shigella from the patient's stool sample, and streak plating is used to ensure that a single species is being cultured. This is because Shigella is a fastidious organism and requires enrichment to grow in quantities that can be used in further tests. Streak plating allows for the separation of individual colonies, which can then be tested for further identification. Gram staining is not necessary for culturing a single species, and pour plates and capsule staining are not mentioned in this case.

The Colonies of Interest in This Case Are the Blue-Green