Prokaryotes are the only organisms able to use atmospheric nitrogen as a nitrogen source.

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Prokaryotes, specifically certain bacteria such as cyanobacteria and some species of bacteria in soil and root systems, have the ability to convert nitrogen gas (N2) from the atmosphere into a usable form for themselves and other organisms in a process called nitrogen fixation. This ability is not present in eukaryotic organisms.

Prokaryotes Are the Only Organisms Able to Use Atmospheric Nitrogen