What growth conditions would you use to enrich for ammonia-oxidizing bacteria?
A) aerobic mineral media with ammonia and bicarbonate incubated in the dark
B) anaerobic mineral media with acetate and ammonia incubated in the dark
C) aerobic mineral media with acetate and ammonia incubated in the dark
D) anaerobic mineral media with ammonia and nitrate incubated in the dark

Question

Quizplus · Accepted Answer

Ammonia-oxidizing bacteria are aerobic, so they require oxygen to grow. They also use ammonia as their energy source, so a mineral media with ammonia would be suitable for enrichment. The presence of bicarbonate would provide a source of carbon for the bacteria. Incubation in the dark would minimize the growth of other organisms that might compete with the ammonia-oxidizing bacteria.

What Growth Conditions Would You Use to Enrich for Ammonia-Oxidizing