Which of the following techniques would be used to identify unknown virulence genes that are only expressed in the host?
A) in situ hybridization of cultured bacteria
B) creating a genomic library of open reading frames of pathogen
C) mutational studies in vitro
D) culture techniques
E) in vivo expression technology

Question

Quizplus · Accepted Answer

In vivo expression technology (IVET) would be the best choice to identify unknown virulence genes that are only expressed in the host. IVET incorporates a promoterless reporter gene that becomes activated upon transcription from a promoter that is only active in vivo (i.e. during infection of the host). This allows for the identification of genes that are specifically expressed during infection and are potentially important for virulence. The other options do not specifically target unknown virulence genes that are only expressed in the host.

Which of the Following Techniques Would Be Used to Identify