A scientist wants to study the effects of summation on graded potentials. Her assistant applies the stimuli, but there is no apparent result. Compose a situation that would explain these results.
A) The stimuli were given, but they did not reach threshold.
B) The stimuli were given too soon after a trial run and the membrane was still in a refractory period.
C) The stimuli caused a hyperpolarization, but graded potentials are only depolarizing.
D) Two stimuli, equal in amplitude, but opposite in polarity were given and when summed they cancelled each other out.
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