Use the following information to answer the next few questions.
A group of six students has taken samples of their own cheek cells, purified the DNA, and used a restriction enzyme known to cut at zero, one, or two sites in a particular gene of interest.
-Their next two steps, in order, should be
A) use of a fluorescent probe for the gene sequence, then electrophoresis.
B) electrophoresis of the fragments followed by autoradiography.
C) electrophoresis of the fragments, followed by the use of a probe.
D) use of a ligase that will anneal the pieces, followed by Southern blotting.
E) use of reverse transcriptase to make cDNA, followed by electrophoresis.

Question

Quizplus · Accepted Answer

The students should first perform electrophoresis of the fragments to separate the DNA pieces based on size. Then, they should use a probe to determine which fragments contain the gene of interest. The probe will bind to complementary sequences in the gene and allow it to be detected.

Use the Following Information to Answer the Next Few Questions