In order to identify a specific restriction fragment using a probe, what must be done?
A) The fragments must be separated by electrophoresis.
B) The fragments must be treated with heat or chemicals to separate the strands of the double helix.
C) The probe must be hybridized with the fragment.
D) The fragments must be separated by electrophoresis and the fragments must be treated with heat or chemicals to separate the strands of the double helix.
E) The fragments must be separated by electrophoresis, the fragments must be treated with heat or chemicals to separate the strands of the double helix, and the probe must be hybridized with the fragment.

Question

Quizplus · Accepted Answer

The fragments must be separated by electrophoresis to separate them based on their size. The fragments must then be treated with heat or chemicals to separate the strands of the double helix so that the probe can bind to the single-stranded DNA. Finally, the probe must be hybridized with the fragment to identify the specific restriction fragment of interest. Thus, option E is the correct choice.

In Order to Identify a Specific Restriction Fragment Using a Probe