Which of the following best describes the complete sequence of steps occurring during every cycle of PCR?
1)The primers hybridize to the target DNA.
2)The mixture is heated to a high temperature to denature the double-stranded target DNA.
3)Fresh DNA polymerase is added.
4)DNA polymerase extends the primers to make a copy of the target DNA.
A) 2, 1, 4
B) 1, 3, 2, 4
C) 3, 4, 1, 2
D) 3, 4, 2
E) 2, 3, 4

Question

Quizplus · Accepted Answer

The correct sequence for a PCR cycle is: denaturation of the double-stranded DNA by heating (2), annealing of primers to the target DNA (1), and extension of the primers by DNA polymerase to synthesize the new DNA strands (4). Fresh DNA polymerase is not added in every cycle; thermostable DNA polymerases that can withstand the high temperatures used in PCR are typically used.

Which of the Following Best Describes the Complete Sequence of Steps