A control cell that is synthesizing a GFP-labeled version of mannosidase II has fluorescence localized in the numerous Golgi complexes of the cell. Normally, this enzyme is synthesized in the endoplasmic reticulum and moves via transport vesicles to the Golgi complex, where it takes up residence. What would an experimental cell look like if it contained an siRNA that led to the absence of one of the proteins involved in the transport of the enzyme from the ER to the Golgi complex? (Select all correct choices)
A)Fluorescent label is not found in the Golgi complex.
B)The GFP-mannosidase II is denatured so there is no fluorescent label anywhere in the cell.
C)Fluorescent label still translocates to the Golgi complex completely.
D)Fluorescent label is found only in the endoplasmic reticulum.

Question

Quizplus · Accepted Answer

The absence of a protein involved in transport from the ER to the Golgi would prevent the enzyme from reaching the Golgi, so fluorescence would not be found in the Golgi (A) and would instead be retained in the ER (D).

A Control Cell That Is Synthesizing a GFP-Labeled Version of Mannosidase