Why is a single-stranded, circular DNA an ineffective template for DNA polymerase?
A)It has a 3'-hydroxyl terminus, but lacks a template.
B)It lacks a 3'-hydroxyl primer, but has a template.
C)It lacks a 3'-hydroxyl group and a template.
D)It has a 3'-hydroxyl group and has a template.
E)It lacks a 5'-hydroxyl group and has a template.

Question

Quizplus · Accepted Answer

DNA polymerase requires a 3'-hydroxyl primer to initiate DNA synthesis. Although the circular DNA has a template, it lacks a primer for DNA polymerase to begin synthesis.

Why Is a Single-Stranded, Circular DNA an Ineffective Template for DNA