Chimeric antigen receptors (CARs)have evolved in molecular structure through three generations of design. Which of the following statements regarding the three generations of CARs is correct?
A)the first generation CARs were the largest most complex molecules
B)the second generation CARs possessed two VH and two VL domains
C)the third generation CARs possessed the most costimulatory domains
D)the third generation CARs possessed three VH and two VL domains

Question

Quizplus · Accepted Answer

The third generation CARs possessed the most costimulatory domains, which was an improvement over the second generation CARs that had only one. The first generation CARs were the smallest and simplest, consisting of only a single-chain variable fragment (scFv) fused to an intracellular signaling domain. The second generation added additional domains such as CD28, 4-1BB, or OX40 for costimulation, and the third generation further increased the number of costimulatory domains to enhance T cell activation and proliferation. Option B is incorrect because the second generation CARs had only one VH and one VL domain. Option D is also incorrect, as CARs typically have one VH and one VL domain.

Chimeric Antigen Receptors (CARs)have Evolved in Molecular Structure Through Three