What is the purpose of the heating cycle during PCR?
A) activates Taq polymerase
B) denatures the DNA
C) facilitates the attachment of RNA primers
D) increases the rate of polymerization by increasing the rate of diffusion of free nucleotides

Question

Quizplus · Accepted Answer

The heating cycle during PCR is primarily used to denature the DNA, separating the double-stranded DNA into single strands so that primers can anneal and DNA polymerase can extend the new DNA strand.

What Is the Purpose of the Heating Cycle During PCR