Successful cotransformation in the yeast two-hybrid system will result in the binding of proteins produced by each yeast expression vector. These fusion proteins function by
A) binding to the lacZ operator, preventing transcription.
B) binding to the lacZ repressor, enabling transcription.
C) changing color in the presence of X-gal.
D) binding to RNA polymerase, preventing transcription.
E) binding to the lacZ upstream activator sequence, enabling transcription.

Question

Quizplus · Accepted Answer

The lacZ upstream activator sequence (UAS) is a DNA sequence that binds to RNA polymerase and enables transcription of the lacZ gene. The fusion proteins produced by the yeast two-hybrid system bind to the UAS, enabling transcription of the lacZ gene.

Successful Cotransformation in the Yeast Two-Hybrid System Will Result in the Binding