You have digested a molecule of DNA and want to identify a specific fragment of interest. The DNA is subjected to gel electrophoresis, but you get two bands that are very close in size. What could you use to determine which band is the correct one?
A) southern blot
B) western blot
C) northern blot
D) eastern blot
E) stain with ethidium bromide

Question

Quizplus · Accepted Answer

Southern blotting is a technique used to identify specific DNA sequences in DNA samples. After electrophoresis, DNA fragments are transferred to a membrane and then probed with a specific sequence of DNA that is complementary to the fragment of interest. This allows for the identification of the specific DNA fragment among others, making it the correct choice for determining which band is the correct one when two bands are very close in size on a gel.

You Have Digested a Molecule of DNA and Want to Identify