Solved

Which of the Following Lists the Steps for CDNA Library

Question 20

Multiple Choice

Which of the following lists the steps for cDNA library preparation in the correct order? I. Partially degrade the mRNA with RNase H
II. Isolate mRNA from a specific cell/ tissue with an oligo- dT primer that hybridizes to poly- A tail
III. Synthesize a strand of cDNA using DNA polymerase with mRNA fragments as primers
IV. Ligate cDNAs into a cloning vector
V. Create a single- stranded DNA molecule complementary to the mRNA using reverse transcriptase


A) II, V, IV, III, I
B) II, V, I, III, IV
C) I, II, III, IV, V
D) II, III, V, I, IV
E) V, III, I, IV, II

Correct Answer:

verifed

Verified

Unlock this answer now
Get Access to more Verified Answers free of charge

Related Questions

Unlock this Answer For Free Now!

View this answer and more for free by performing one of the following actions

qr-code

Scan the QR code to install the App and get 2 free unlocks

upload documents

Unlock quizzes for free by uploading documents