The following steps are used to produce monoclonal antibodies. What is the fourth step?
A) Culture the hybridoma in a selective medium.
B) A B cell is activated to produce antibodies.
C) Isolate antibody-producing B cells.
D) Vaccinate a mouse.
E) Fuse a B cell to a myeloma cell.

Question

Quizplus · Accepted Answer

The fourth step in producing monoclonal antibodies is to fuse a B cell to a myeloma cell, creating a hybridoma. This follows the activation of a B cell, isolation of antibody-producing B cells, and vaccination of a mouse.

The Following Steps Are Used to Produce Monoclonal Antibodies