In the serological method for tissue typing, if human cells expressing HLA -I are mixed with anti-HLA-I, complement, and trypan blue, what would indicate the cells are HLA -I?
A) Enzyme is released from neutrophils.
B) Trypan blue enters the cells.
C) Cells are damaged by complement.
D) Anti-HLA antibodies bind to HLAs on lymphocytes.
E) None of the answers are correct; the T cells are missing.

Question

Quizplus · Accepted Answer

If the cells express HLA-I and are bound by anti-HLA-I antibodies, the complement system will be activated, leading to cell lysis. Trypan blue will enter the damaged cells, indicating they are HLA-I positive.

In the Serological Method for Tissue Typing, If Human Cells