For polymerase chain reaction to occur,
A) primers must hydrogen-bond to the template DNA
B)all DNA fragments must be identical.
C) the DNA must remain double stranded.
D)a sticky end must be available for the ligase enzyme to function.
E)all of these must occur.

Question

For polymerase chain reaction to occur, 
A) primers must hydrogen-bond to the template DNA
B)all DNA fragments must be identical. 
C) the DNA must remain double stranded.
D)a sticky end must be available for the ligase enzyme to function.
E)all of these must occur.

Quizplus · Accepted Answer

In PCR, primers are essential for initiating the amplification process. They are designed to specifically bind to the target DNA sequence, thereby providing a starting point for the polymerase enzyme to extend new complementary strands. The primers hydrogen-bond to the template DNA, allowing the polymerase to add new nucleotides and replicate the target sequence. The other options (B, C, and D) are not correct since PCR can amplify a wide range of DNA fragments, the DNA must be single-stranded during amplification, and PCR does not require the use of ligase enzymes. Therefore, the best choice is A.

For Polymerase Chain Reaction to Occur