All of the following are associated with the formation of a functional miRNA except
A) lack of an open reading frame (ORF).
B) 5' GTP cap and 3' poly A tail.
C) stem-loop structures.
D) translation on ribosomes.
E) RNA pol II promoter.

Question

Quizplus · Accepted Answer

Formation of a functional miRNA does not involve translation on ribosomes, as miRNAs are single-stranded RNA molecules that do not code for proteins. Instead, they regulate gene expression by binding to target mRNAs and inhibiting their translation. The other choices are all associated with miRNA biogenesis, as stem-loop structures are the precursor structures for miRNAs, lack of an ORF is a characteristic of non-coding RNAs like miRNAs, RNA pol II promoter is involved in transcription of miRNA genes, and the 5' GTP cap and 3' poly A tail are added post-transcriptionally during miRNA processing.

All of the Following Are Associated with the Formation of a Functional