What is the correct workflow for pyrosequencing?
A) ligation,hybridization,light emission,amplification
B) hybridization,ligation,amplification,light emission
C) ligation,hybridization,ligation,amplification
D) ligation,hybridization,amplification,light emission
E) light emission,hybridization,amplification,ligation

Question

Quizplus · Accepted Answer

The correct workflow for pyrosequencing is ligation of a sequencing adaptor to the DNA fragments, followed by hybridization of these fragments to a sequencing primer, amplification by PCR using a universal primer, and detection of light emissions as nucleotides are incorporated into the growing strand. Therefore, the correct workflow is ligation, hybridization, amplification, and light emission as in choice D.

What Is the Correct Workflow for Pyrosequencing