Which of the following is not useful in a complementation test? A) recessive alleles B) sexual reproduction C) pure breeding lines D) F₁ progeny E) alleles dominant to wild-type

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A complementation test is used to determine if two mutations are in the same gene or in different genes. It relies on having recessive alleles (A), sexual reproduction (B) to bring together alleles from different parents, and pure breeding lines (C) to ensure that the genetic background is consistent. F₁ progeny (D) could potentially be used in such a test depending on the context. However, alleles that are dominant to wild-type (E) are not useful in a complementation test because the test requires recessive mutations to see if they can complement each other when brought together in the same organism. Dominant alleles would mask the effect of other alleles, making it impossible to determine complementation.

Which of the Following Is Not Useful in a Complementation