You grow a barcoded yeast deletion set at 4°C and then a copy of the same set at 25°C.Barcodes from the 4°C sample and 25°C are labeled with a green fluorochrome and a red fluorochrome,respectively.How will you identify genes required for growth specifically at cold temperatures?
A) separate the PCR products on an agarose gel and look for red fluorescent bands
B) separate the PCR products on an agarose gel and look for yellow fluorescent bands
C) hybridize the products to a microarray and look for red spots
D) hybridize the products to a microarray and look for green spots
E) separate the PCR products on an agarose gel and look for green fluorescent bands

Question

Quizplus · Accepted Answer

Hybridizing the PCR products to a microarray and looking for red spots will identify genes required for growth specifically at cold temperatures because the red fluorochrome labels the 25°C sample. If a gene is required for growth at 4°C, its deletion would result in reduced or no growth at 4°C, thus less green fluorescence and relatively more red fluorescence from the 25°C sample where the yeast can grow normally.

You Grow a Barcoded Yeast Deletion Set at 4°C and Then