Which of the following is used in site-directed mutagenesis?
A) alkylating agent
B) hydroxylating agent
C) deaminating agent
D) synthetic oligonucleotide
E) intercalating agent

Question

Quizplus · Accepted Answer

Site-directed mutagenesis involves the introduction of specific mutations into a DNA sequence, typically by using a synthetic oligonucleotide that is complementary to the target sequence, but contains the desired mutation. Alkylating, hydroxylating, and deaminating agents are not commonly used in site-directed mutagenesis. Intercalating agents can be used to detect mutations, but not to introduce them.

Which of the Following Is Used in Site-Directed Mutagenesis