Use the following information to answer the question.
Suzanne Simard and colleagues knew that the same mycorrhizal fungal species could colonize multiple types of trees. They wondered if the same fungal individual would colonize different trees, forming an underground network that potentially could transport carbon and nutrients from one tree to another (S. Simard et al. 1997. Net transfer of carbon between mycorrhizal tree species in the field. Nature 388:579-82).
Pots containing seedlings of three different tree species were set up and grown under natural conditions for three years (Fig.A). Two of the three species (Douglas fir, birch) could form ectomycorrhizal connections with the same fungal species, but the third species (cedar) could not form an ectomycorrhizal connection with the fungal species. In some of the pots, the researchers placed airtight bags over the Douglas fir and birch seedlings and injected carbon dioxide made from carbon-13 into the bags with the Douglas fir and carbon dioxide made from carbon-14 into the bags with the birch. (¹³C and ¹⁴C are different isotopes of carbon that can be detected and measured by researchers.) As the seedlings photosynthesized, the carbon dioxide was converted into sugars that could be tracked and measured by the researchers. The researchers measured whether the sugars in each plant contained only the carbon isotope that was in the air of their plastic bag or also the carbon isotope from the air around the other plant.

Figure A
Which of the following results would support Simard et al.'s (1997) hypothesis that fungi can move carbon from one plant to another? [Hypothesis: Sugars made by one plant during photosynthesis can travel through a mycorrhizal fungus and be incorporated into the tissues of another plant.]
A) Carbon-14 is found in the birch seedling's tissues and carbon-13 in the Douglas fir.
B) Carbon-14 is found in the Douglas fir seedling's tissues and carbon-13 in the birch.
C) Either carbon-13 or carbon-14 is found in the fungal tissues.
D) Either carbon-13 or carbon-14 is found in the cedar seedling's tissues.

Question

Use the following information to answer the question.
Suzanne Simard and colleagues knew that the same mycorrhizal fungal species could colonize multiple types of trees. They wondered if the same fungal individual would colonize different trees, forming an underground network that potentially could transport carbon and nutrients from one tree to another (S. Simard et al. 1997. Net transfer of carbon between mycorrhizal tree species in the field. Nature 388:579-82).
Pots containing seedlings of three different tree species were set up and grown under natural conditions for three years (Fig.A). Two of the three species (Douglas fir, birch) could form ectomycorrhizal connections with the same fungal species, but the third species (cedar) could not form an ectomycorrhizal connection with the fungal species. In some of the pots, the researchers placed airtight bags over the Douglas fir and birch seedlings and injected carbon dioxide made from carbon-13 into the bags with the Douglas fir and carbon dioxide made from carbon-14 into the bags with the birch. (¹³C and ¹⁴C are different isotopes of carbon that can be detected and measured by researchers.) As the seedlings photosynthesized, the carbon dioxide was converted into sugars that could be tracked and measured by the researchers. The researchers measured whether the sugars in each plant contained only the carbon isotope that was in the air of their plastic bag or also the carbon isotope from the air around the other plant.

Figure A
Which of the following results would support Simard et al.'s (1997) hypothesis that fungi can move carbon from one plant to another? [Hypothesis: Sugars made by one plant during photosynthesis can travel through a mycorrhizal fungus and be incorporated into the tissues of another plant.]
A) Carbon-14 is found in the birch seedling's tissues and carbon-13 in the Douglas fir. 
B) Carbon-14 is found in the Douglas fir seedling's tissues and carbon-13 in the birch. 
C) Either carbon-13 or carbon-14 is found in the fungal tissues. 
D) Either carbon-13 or carbon-14 is found in the cedar seedling's tissues.

Quizplus · Accepted Answer

The experiment involved injecting different isotopes of carbon (¹³C and ¹⁴C) into the bags with the seedlings of different tree species. If the mycorrhizal fungus network can transport carbon from one plant to another, then the sugars made by one plant should be detected in the tissues of another plant that shares the same fungal individual. In this case, if carbon-14 is found in the Douglas fir seedling's tissues and carbon-13 in the birch, it would suggest that the carbon made by the birch has been transferred through the fungus to the Douglas fir. The other choices do not directly support the hypothesis or are not relevant to the experimental design.

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