Using Modern Techniques of Sequencing by Synthesis and the Shotgun

Using modern techniques of sequencing by synthesis and the shotgun approach, sequences are assembled into chromosomes by ________.

A) placing them on previously generated genetic maps
B) cloning them into plasmid vectors
C) computer analysis looking for sequence overlaps
D) cloning them into plasmid vectors, placing them on previously generated genetic maps, followed by computer analysis looking for sequence overlaps