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Organisms share many conserved core processes and features, including transcription and translation, using a uniform genetic code. Scientists have used these shared processes and features in biotechnology. For example, for the process of some transformations, a plasmid is constructed when a eukaryotic gene of interest is added with an antibiotic resistant gene such as beta-lactamase, which is used for ampicillin resistance. This plasmid is then inserted into a prokaryotic bacterial cell, such as E. coli, through a transformation process that leads to the production of the product protein from the eukaryotic organism. To culture the bacteria and obtain the protein product, the bacteria must grow.
Select the appropriate condition to determine if the plasmid has entered the E. coli bacterial cell.
A) nutrient broth to which no antibiotic has been added
B) water to which ampicillin has been added
C) nutrient broth to which ampicillin has been added
D) nutrient broth to which other resistant bacteria have been added

Question

Quizplus · Accepted Answer

The addition of ampicillin to the nutrient broth will only allow the growth of bacteria that have successfully taken up the plasmid with the antibiotic resistant gene. Therefore, if the bacteria grow in the nutrient broth with ampicillin, it indicates that the plasmid has been successfully transformed into the E. coli bacterial cell.

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