Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot, one generally _.
A) examines amino acid substitutions with radioactive probes
B) ligates DNA with DNA ligase
C) hybridizes filter -bound RNA with a DNA probe
D) hybridizes filter -bound DNA with a DNA probe
E) cleaves RNA with restriction endonucleases

Question

Quizplus · Accepted Answer

In a Southern blot, DNA fragments are separated by gel electrophoresis, transferred to a membrane, and then hybridized with a labeled DNA probe to detect specific DNA sequences.

Nucleic Acid Blotting Is Widely Used in Recombinant DNA Technology