An epidemiologist is investigating a new disease and observes what appear to be bacteria inside tissue cells in clinical samples from victims. The scientist wants to try to isolate the bacteria in the lab. What culture conditions are most likely to be successful?
A) culturing on blood agar plates
B) incubation in an anaerobic culture system
C) inoculation of EMB plates
D) inoculation of a minimal medium broth
E) inoculation of cell cultures

Question

Quizplus · Accepted Answer

The bacteria are observed inside tissue cells, suggesting they are intracellular bacteria. Therefore, inoculation of cell cultures, which can provide the necessary environment for intracellular growth, is most likely to be successful.

An Epidemiologist Is Investigating a New Disease and Observes What