An in vitro transcription system that contains a bacterial gene initiates transcription from random points on the DNA. Which of the following proteins MOST likely is missing from the reaction?
A) sigma factor
B) rho factor
C) RNA polymerase II
D) TATA-binding protein (TBP)
E) TFIID

Question

Quizplus · Accepted Answer

The sigma factor is a component of bacterial RNA polymerase that is essential for the initiation of transcription at specific promoter sites. Without it, transcription may start at random points on the DNA.

An in Vitro Transcription System That Contains a Bacterial Gene