Choose the CORRECT order of procedure in performing chromatin immunoprecipitation (ChIP). 1. DNA sequencing and fragment identification
2) Removal of crosslinking and separation of DNA and proteins
3) Crosslinking proteins and chromosomes via UV
4) Antibody incubation for immunoprecipitation
5) Protein degradation via proteases
6) Cell lysis and chromosome fragmentation
A) 1, 3, 2, 4, 5, 6
B) 2, 6, 5, 1, 3, 4
C) 4, 3, 2, 5, 1, 5
D) 3, 6, 4, 2, 5, 1
E) 5, 1, 3, 6, 4, 2

Question

Quizplus · Accepted Answer

The correct order is: crosslinking proteins and chromosomes (3), cell lysis and chromosome fragmentation (6), antibody incubation for immunoprecipitation (4), removal of crosslinking and separation of DNA and proteins (2), protein degradation via proteases (5), and finally DNA sequencing and fragment identification (1).

Choose the CORRECT Order of Procedure in Performing Chromatin Immunoprecipitation