The normal functions of Spo11 and Cas9 enzymes are different because
A)Spo11 produces double-strand breaks during meiosis, whereas Cas9 functions during mitosis.
B)Spo11 produces site-specific double-strand breaks during meiosis, whereas Cas9 breaks DNA at random genomic locations.
C)Spo11 produces double-strand breaks in many genomic locations during meiosis, whereas Cas9 is targeted to specific sequences by a CRISPR RNA.
D)Spo11 produces double-strand breaks at sequences targeted by RNAs expressed during meiosis, whereas Cas9 has the ability to recognize DNA sequences without an RNA.
E)Spo11 is unique to yeast, whereas Cas9 occurs in most higher eukaryotes.

Question

Quizplus · Accepted Answer

Spo11 is involved in initiating homologous recombination during meiosis by creating double-strand breaks in DNA across many locations, which is crucial for the exchange of genetic material between homologous chromosomes. Cas9, on the other hand, is an enzyme used in CRISPR technology for genome editing and is directed to specific DNA sequences by guide RNAs, allowing for precise cuts at targeted locations.

The Normal Functions of Spo11 and Cas9 Enzymes Are Different