Yeast research with autonomously replicating sequences (ARS), along with digestion of chromatin with DNase I, have led scientists to determine that
A)DNA is not protected inside a nucleosome.
B)ARSs are really plasmids.
C)ARSs bond irreversibly to replication enzymes.
D)origins of replication are accessible regions of DNA devoid of nucleosomes.

Question

Quizplus · Accepted Answer

Origins of replication, such as ARSs in yeast, are accessible regions of DNA because they are devoid of nucleosomes. This accessibility is necessary for replication machinery to bind and initiate DNA replication. The experiments involving DNase I digestion of chromatin have shown that DNA regions covered by nucleosomes are protected from DNase I digestion, whereas accessible regions, like origins of replication, are more susceptible, indicating they are not covered by nucleosomes.

Yeast Research with Autonomously Replicating Sequences (ARS), Along with Digestion