Place the following steps for cloning DNA in the correct order:
1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product
A)1,2,3,4,5
B)5,3,2,4,1
C)2,5,3,1,4
D)3,2,4,1,5

Question

Place the following steps for cloning DNA in the correct order:
 1 - use vector to deliver new rDNA to bacterial or other cells
2 - use restriction enzymes to cut a plasmid and add the desired gene
3 - isolate and cut out a desired gene using restriction enzymes
4 - use DNA ligase to seal the new gene
5 - allow bacterial cells to replicate and produce desired product
A)1,2,3,4,5
B)5,3,2,4,1
C)2,5,3,1,4
D)3,2,4,1,5

Quizplus · Accepted Answer

First, isolate and cut out the desired gene (3), then cut a plasmid and add the gene (2), seal the gene with DNA ligase (4), deliver the rDNA to cells (1), and finally allow cells to replicate (5).

Place the Following Steps for Cloning DNA in the Correct