In experimental tests of enzyme evolution,a gene encoding an enzyme was subjected to multiple cycles of random mutagenesis and selection for altered substrate specificity.The resulting enzyme with altered substrate specificity had multiple amino acid changes.Where in the enzyme might these amino acid changes be located?
A)only in or near the active site
B)only in the hydrophobic interior of the folded protein
C)only on surface sites distant from the active site
D)in or near the active site,in the hydrophobic interior,and/or at surface sites distant from the active site

Question

Quizplus · Accepted Answer

The amino acid changes could be located in or near the active site, as this is where interactions with the substrate occur. They could also be in the hydrophobic interior, as this region helps to maintain protein folding and stability. Additionally, changes could occur at surface sites distant from the active site, as these can affect protein-protein interactions and overall stability. Therefore, the changes could be located in any of these regions, making D the best choice.

In Experimental Tests of Enzyme Evolution,a Gene Encoding an Enzyme