During DNA replication in E.coli,which of the following enzymes removes the RNA nucleotides from the primer and adds equivalent DNA nucleotides to the 3' end of Okazaki fragments?
A)helicase
B)DNA polymerase III
C)ligase
D)DNA polymerase I

Question

Quizplus · Accepted Answer

DNA polymerase I, also known as pol I, is responsible for removing RNA primers during DNA replication in E.coli and replacing them with DNA nucleotides. This process is called nick translation, where the 5' to 3' exonuclease activity of pol I removes the RNA primer, and its 5' to 3' polymerase activity adds DNA nucleotides to the 3' end of the Okazaki fragments. Helicase unwinds the DNA, DNA polymerase III extends the new strand and ligase seals the gaps between the fragments, but none of these enzymes remove RNA primers during replication.

During DNA Replication in E