Which of the following would not be true of cDNA produced using human brain tissue as the starting material?
A)It could be amplified by the polymerase chain reaction.
B)It was produced from pre-mRNA using reverse transcriptase.
C)It could be labeled and used as a probe to detect genes expressed in the brain.
D)It lacks the introns of the pre-mRNA.

Question

Quizplus · Accepted Answer

cDNA is produced using reverse transcriptase to convert RNA (in this case, pre-mRNA) into single-stranded DNA. Therefore, choice B is incorrect as it states that cDNA was produced from pre-mRNA using reverse transcriptase, which is not accurate.

Which of the Following Would Not Be True of CDNA