Which of the following techniques involves reverse transcriptase,PCR amplification,and gel electrophoresis?
A)DNA microarray assays
B)RT-PCR
C)in situ hybridization
D)nucleic acid hybridization

Question

Quizplus · Accepted Answer

Reverse transcriptase is used to convert RNA to cDNA, which is then PCR amplified and run on a gel to visualize the product. This is known as RT-PCR. DNA microarray assays involve hybridizing labeled nucleic acids to a chip containing many probes, in situ hybridization involves using a labeled probe to detect nucleic acids in situ, and nucleic acid hybridization involves annealing complementary strands of nucleic acids. None of these methods involve the use of reverse transcriptase and PCR amplification.

Which of the Following Techniques Involves Reverse Transcriptase,PCR Amplification,and Gel