Scientists Have Identified Two Major Branches of Prokaryotic Evolution

Scientists have identified two major branches of prokaryotic evolution.What was the basis for dividing prokaryotes into two domains?

A) microscopically observed staining characteristics of the cell wall
B) metabolic characteristics such as the production of methane gas
C) metabolic characteristics such as chemoautotrophy and photosynthesis
D) genetic characteristics such as ribosomal RNA sequences
E) ecological characteristics such as the ability to survive in extreme environments