The $\beta$-galactosidase gene that is inserted into plasmid cloning vectors is used to
A) cleave the DNA in a sequence-specific fashion.
B) disrupt the antibiotic-resistance gene, making it nonfunctional.
C) determine if the cloning has been successful.
D) protect the bacteria from bacteriophage infection.

Question

Quizplus · Accepted Answer

The F1F1F1S1F1F1F10-galactosidase gene is used as a reporter gene to determine if the cloning has been successful. When the cloned DNA is inserted into the plasmid and the plasmid is inserted into the bacteria, the F1F1F1S1F1F1F10-galactosidase gene is also inserted. If the cloning is successful, the bacteria will produce β-galactosidase, which can then be detected using a colorimetric assay. This allows researchers to determine if the insertion of the foreign DNA into the plasmid was successful.

The β\betaβ -Galactosidase Gene That Is Inserted into Plasmid Cloning Vectors Is

The $\beta$ -Galactosidase Gene That Is Inserted into Plasmid Cloning Vectors Is