In the Anfinsen experiment with the unfolding of RNaseA,what order could the chemical reagents be removed in order to achieve an inactive protein?
A) Remove denaturant first and reductant second.
B) Simultaneously remove denaturant and reductant.
C) Remove reductant first, denaturant second, and then finally add back reductant.
D) Remove reductant first and denaturant second.

Question

Quizplus · Accepted Answer

In the Anfinsen experiment, removing the reductant first and denaturant second is the best choice as this would result in the protein being left in its oxidized, disulfide-bonded inactive form. This is because the disulfide bonds are formed between cysteine residues in a protein via oxidation, and the reducing agent would have been used to break these bonds and disrupt the protein structure. Removing the denaturant alone would not be sufficient to inactivate the protein. Similarly, simultaneous removal of both denaturant and reductant may not yield the same result as sequential removal. Finally, adding back the reductant after removing both the denaturant and reductant would only serve to activate the protein again by breaking the disulfide bonds.

In the Anfinsen Experiment with the Unfolding of RNaseA,what Order