Which of the following is an assumption made when using Michaelis-Menten kinetics? A) The conversion of E + P $\rightarrow$ ES does not occur. B) k₁ k₂ C) v₀ = v_max at low [S] D) The conversion of EP $\rightarrow$ E + P is rapid.

Question

Which of the following is an assumption made when using Michaelis-Menten kinetics? A) The conversion of E + P $\rightarrow$ ES does not occur. B) k₁ > k₂ C) v₀ = v_max at low [S] D) The conversion of EP $\rightarrow$ E + P is rapid.

Quizplus · Accepted Answer

The assumption in Michaelis-Menten kinetics that the conversion of the enzyme-product complex (EP) back to the enzyme (E) and product (P) is rapid is crucial for the derivation of the Michaelis-Menten equation. This assumption allows for the simplification of the kinetic analysis by assuming that the product formation step does not limit the overall rate of the reaction.

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