How can freeze-fracture be used to determine the orientation of a protein in a membrane?
A)Freeze-fracture allows a cell to be cleaved in between the lipid bilayer,splitting the plasma membrane into two layers.
B)Freeze-fracture allows a cell to be cleaved in such a way as to preserve the embedded proteins.
C)Freeze-fracture cuts the membrane in protein-sized components for viewing under an electron microscope.

Question

Quizplus · Accepted Answer

Freeze-fracture involves splitting the lipid bilayer, which can reveal the distribution and orientation of proteins within the membrane by examining the exposed surfaces.

How Can Freeze-Fracture Be Used to Determine the Orientation of a Protein