Which of the following two enzymes are needed to construct a cDNA library?
A)Reverse transcriptase and DNA ligase
B)Reverse transcriptase and Taq polymerase
C)DNA ligase and Taq polymerase
D)Taq polymerase and RNA polymerase

Question

Quizplus · Accepted Answer

A cDNA library is constructed using reverse transcriptase, which converts RNA to cDNA, and DNA ligase, which joins the cDNA fragments into a library. Taq polymerase is used in PCR, not in cDNA library construction. RNA polymerase is involved in transcription, but not in constructing a cDNA library.

Which of the Following Two Enzymes Are Needed to Construct