What is necessary to generate a genomic DNA library?
A)Many overlapping fragments of the genome with at least one copy of every DNA sequence in the organism.
B)A single fragment that contains at least one copy of the DNA sequence in the organism's genome.
C)Many fragments of the genome with at least one copy of every DNA sequence in the organism.
D)Many overlapping fragments of the genome with at least one copy of every DNA sequence in the organism's genome.
E)Many fragments of the genome with at least one copy of every DNA sequence in the organism's genome.

Question

Quizplus · Accepted Answer

A genomic DNA library requires many overlapping fragments of the genome in order to ensure that every DNA sequence in the organism's genome is represented at least once. These fragments can be generated by cutting the genomic DNA into smaller pieces using restriction enzymes or mechanical shearing, and then cloning these fragments into a suitable vector such as a bacterial artificial chromosome (BAC) or a phage vector. By generating many overlapping fragments, the library can be screened for specific genes or sequences of interest, and the full genome sequence can potentially be reconstructed.

What Is Necessary to Generate a Genomic DNA Library