A common first separation technique in 2D gels is isoelectric focusing.What property is isoelectric focusing taking advantage of?
A)charge
B)amino acid content
C)the pH at which the overall charge is zero
D)size

Question

Quizplus · Accepted Answer

Isoelectric focusing separates proteins based on their isoelectric point (pI), the pH at which their net charge is zero. By subjecting proteins to a pH gradient created using ampholytes (molecules with both acidic and basic groups), proteins migrate until they reach their pI, where they become electrically neutral and stop migrating. This creates distinct bands of proteins along the pH gradient, allowing for separation and analysis. Therefore, the property that isoelectric focusing takes advantage of is the pH at which the overall charge is zero.

A Common First Separation Technique in 2D Gels Is Isoelectric