Nested PCR is very sensitive and specific because
A) it uses more than one DNA polymerase to make three to four times the amount of PCR products than standard PCR.
B) the assay itself basically serves as a form of internal control and ensures specificity.
C) the assay will act as a form of external control, ensuring specificity and sensitivity.
D) the fluorochrome that is attached to the primers ensures specificity of the nucleotides that are attached to the template.

Question

Quizplus · Accepted Answer

Nested PCR uses two sets of primers to amplify a specific target sequence. By using the product of the first PCR as the template for the second PCR, specificity is greatly increased because it ensures that only the target sequence is amplified. This serves as an internal control, making the assay more specific and sensitive. The use of multiple DNA polymerases (A) or fluorochromes (D) are not specific features of nested PCR, and an external control (C) may be used but is not unique to nested PCR.

Nested PCR Is Very Sensitive and Specific Because