In a hybridization reaction, the pH affects
A) the probe's nucleic acids so that they do not lose their hydrogen atoms.
B) the breakdown of the oligonucleotide pieces.
C) the ability of the mismatches between the probe and the target to duplex.
D) the stability of double-stranded nucleic acid molecules in solution.

Question

Quizplus · Accepted Answer

The pH affects the stability of double-stranded nucleic acid molecules in solution because it influences the hydrogen bonding between the nucleotide bases, which is crucial for the stability of the double helix structure.

In a Hybridization Reaction, the PH Affects